Abstract
The present study evaluated the antinociceptive effect of (1→3),(1→6)-linked β-glucan
(GL) isolated from Pleurotus pulmonarius (Fr.) Quel. in mice and its possible mechanism of action. Intraperitoneal administration
of GL inhibited glutamate-induced licking with an ID50 of 0.34 mg/kg and inhibition of 96% ± 3%. The treatment of animals with GL (1 mg/kg
i.p.) inhibited nociception induced by intrathecal injection of N-methyl-D-aspartic
acid, α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid, kainate and interleukin
-1β in 67% ± 13%, 89% ± 11%, 74% ± 9%, and 75% ± 7%, respectively, but not the nociceptive
response induced by (±)-1-aminocyclopentane-trans-1,3-dicarboxylic acid, substance
P, and tumor necrosis factor-α. Moreover, GL (30 mg/kg i.p.) also reduced mechanical
allodynia caused by partial sciatic nerve ligation for 2 hours, with inhibition of
47% ± 10% observed 0.5 hours after treatment. When given chronically (twice a day)
over 7 days, GL reversed the mechanical allodynia caused by partial sciatic nerve
ligation (inhibition of 45% ± 13% to 60% ± 8%). Interestingly, GL did not affect the
locomotor activity of mice in an open field test with doses that produce antinociceptive
effects. Our findings show that GL inhibits acute and neuropathic pain in mice through
mechanisms that involve the inhibition of ionotropic glutamate receptors and the interleukin
-1β pathway.
Perspective
This article presents the antinociceptive activity of GL in acute and neuropathic
pain with participation of ionotropic glutamate receptors and pro-inflammatory cytokines
(interleukin-1β). After further experiments, this compound may represent a new pharmacological
agent for the treatment of clinical pain.
Key words
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Article info
Publication history
Published online: April 26, 2010
Accepted:
January 11,
2010
Received in revised form:
November 20,
2009
Received:
August 14,
2009
Footnotes
Supported by Brazilian funding agencies CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), FAPESC (Fundação de Apoio à Pesquisa Científica Tecnológica do Estado de Santa Catarina), Fundação Araucária, and FINEP [Financiadora de Estudos e Projetos, Rede Instituto Brasileiro de Neurociências (IBN-Net).
Identification
Copyright
© 2010 American Pain Society. Published by Elsevier Inc. All rights reserved.
