Abstract
CXBH mice, known as an “opioid receptor-rich” strain, are a recombinant inbred mouse
strain established by crossing the C57BL/6By and BALB/cBy strains. In the present
study, we investigated nociceptive and antinociceptive sensitivity in CXBH mice and
elucidated the underlying molecular mechanisms. CXBH mice exhibited slightly higher
morphine-induced antinociception compared with C57BL/6J and BALB/cBy mice in the hot-plate
test but not tail-flick test. CXBH mice exhibited a marked reduction of nociceptive
sensitivity, regardless of the type of nociceptive stimulus, with the exception of
tail stimulation. Changes in gene expression that corresponded to reduced nociceptive
sensitivity in the brains of CXBH mice were observed in 62 transcripts, including
pain- and analgesia-related transcripts, in a whole-genome expression assay. The total
mRNA expression of opioid receptors was higher in CXBH mice than in C57BL/6J and BALB/cBy
mice. However, the expression levels of MOR-1 mRNA, a major transcript of the μ opioid receptor gene, were not different among
the C57BL/6J, BALB/cBy, and CXBH strains. In conclusion, supraspinal nociceptive responses
were reduced in the CXBH mouse strain, and the expression levels of transcripts were
altered in the brain of this strain.
Perspective
This article presents the nociceptive and antinociceptive properties of CXBH recombinant
inbred mice and gene expression differences that may underlie nociceptive tolerance
in the strain. The CXBH mouse strain may be a useful animal model to investigate the
molecular basis of individual differences in supraspinal pain sensitivity.
Key words
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Article info
Publication history
Published online: April 15, 2013
Accepted:
January 29,
2013
Received in revised form:
January 17,
2013
Received:
November 29,
2012
Footnotes
This research was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (20390162, 22790518, 23390377), Japanese Ministry of Health, Labour and Welfare (H21-3jigan-ippan-011), and Smoking Research Foundation.
There are no conflicts of interest in the article.
Identification
Copyright
© 2013 Published by Elsevier Inc.
