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Address reprint requests to Kelly M. Naugle, PhD, Department of Kinesiology, Indiana University Purdue University Indianapolis, 901 West New York St., Indianapolis, IN 46202.
Younger adults showed greater exercise-induced hypoalgesia (EIH) than older adults.
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Age differences in EIH varied across experimental pain induction methods.
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Older adults did not exhibit increased pain perception after acute exercise.
Abstract
Laboratory-based studies show that acute aerobic and isometric exercise reduces sensitivity to painful stimuli in young healthy individuals, indicative of a hypoalgesic response. However, little is known regarding the effect of aging on exercise-induced hypoalgesia (EIH). The purpose of this study was to examine age differences in EIH after submaximal isometric exercise and moderate and vigorous aerobic exercise. Healthy older and younger adults completed 1 training session and 4 testing sessions consisting of a submaximal isometric handgrip exercise, vigorous or moderate intensity stationary cycling, or quiet rest (control). The following measures were taken before and after exercise/quiet rest: 1) pressure pain thresholds, 2) suprathreshold pressure pain ratings, 3) pain ratings during 30 seconds of prolonged noxious heat stimulation, and 4) temporal summation of heat pain. The results revealed age differences in EIH after isometric and aerobic exercise, with younger adults experiencing greater EIH compared with older adults. The age differences in EIH varied across pain induction techniques and exercise type. These results provide evidence for abnormal pain modulation after acute exercise in older adults.
Perspective
This article enhances our understanding of the influence of a single bout of exercise on pain sensitivity and perception in healthy older compared with younger adults. This knowledge could help clinicians optimize exercise as a method of pain management.
Substantial evidence supports the use of exercise as an effective tool to reduce pain, and it is often recommended as an adjunct therapy in the treatment of chronic pain.
In addition, a single bout of exercise influences the experience of pain. In healthy young adults, acute aerobic and isometric exercise temporarily reduces pain sensitivity, a phenomenon termed exercise-induced hypoalgesia (EIH).
However, many individuals with chronic pain (ie, fibromyalgia, neuropathic pain) do not experience EIH, and pain sensitivity and perception are often temporarily exacerbated after acute exercise.
As regular exercise becomes an important component of the multidisciplinary treatment recommended for persistent pain in older adults, a comprehensive understanding of how acute exercise influences pain perception in this age group is important to optimize exercise as a method of pain management.
The experience of pain is modulated by complex endogenous systems that both facilitate and inhibit pain.
Substantial evidence from psychophysical tests (ie, condition pain modulation [CPM], offset analgesia) indicates that dysfunction of pain inhibitory systems increases with age.
A recent study revealed that endogenous pain inhibitory capacity, as shown on the CPM test, predicted the magnitude of pain reduction after acute isometric exercise.
Thus, participants who had a poor pain inhibitory capacity were more likely to experience a hyperalgesic response after isometric exercise. Despite this evidence, little research has explored changes in pain sensitivity and perception after acute exercise in healthy older adults, a cohort who typically show poor pain inhibitory capacity. One of the only studies to address this topic
investigated the effect of isometric contractions that varied in intensity and duration on pressure pain perception in older and younger adults. Both older and younger adults showed reductions in pain after the isometric contractions; however, the EIH effect was smaller in older adults. To the best of our knowledge, no studies have examined EIH after aerobic exercise in older adults.
The primary purpose of the current study was to examine age differences in EIH after submaximal isometric exercise and moderate and vigorous aerobic exercise. Several studies have shown that experimental pain measures correlate only moderately across stimulus modalities and tests
; thus, we tested for EIH using a multimodal pain assessment. We assessed changes in threshold and suprathreshold pressure pain, temporal summation (TS) of pain, and pain perception during a prolonged heat pain test before and immediately after exercise. These pain measures likely represent distinct dimensions of pain perception that may be under the influence of different mechanisms.
We hypothesized that EIH would be reduced in older compared with younger adults across all forms of acute exercise and pain tests.
Methods
Participants
Participants were 25 (age range, 19–30 years; average age, 21.7 ± 4.1 years; 14 females) healthy young adults and 18 (age range, 55–74 years; average age, 63.7 ± 6.6 years; 9 females) older adults. Studies show that adults 55 years and older have reduced capacity for pain inhibition
; therefore, we included adults 55 years and older in our older adult group. The younger group included 16 whites, 3 Asian Americans, and 6 Hispanic Americans. The older group included 15 whites, 1 Asian American, and 2 Hispanic Americans. Detailed results of the data on the younger adults have been published previously.
A power analysis using G Power 3.1.5 (Heinrich Heine University, Dusseldorf Germany) was used to estimate the sample size needed for detecting a session by age group interaction for the outcome measures in a mixed model design. With the significance level set at .05, power at .80, a .5 correlation among repeated measures, and the effect size (ES) for differences between groups estimated to be moderate, the power analyses determined that a total of 34 participants (17 per group) would yield a power of .81.
Participants were recruited through posted advertisements in the local community. Exclusion criteria included: 1) current use of narcotics or tobacco products, 2) uncontrolled hypertension, 3) neurologic disease with significant changes in somatosensory and pain perception at intended stimulation sites, 4) the known presence of or any signs or symptoms of cardiovascular disease, pulmonary disease, or metabolic disease, 5) serious psychiatric conditions (eg, schizophrenia and bipolar disorder), and 6) current use of opioids. Younger adults were excluded if they were not physically ready to exercise without a medical examination as indicated by the Physical Activity Readiness Questionnaire (PAR-Q).
Older adults had to obtain physician approval from their primary care physician to participate in the study. During the health history interview, no participants indicated that they were regularly taking pain medications or reported chronic pain. Session exclusion criteria included active infectious disease or febrile condition (eg, sinusitis, influenza), severe uncontrolled hypertension, use of caffeinated drinks, or any pain medications before the experimental sessions.
Procedures
The university institutional review board (IRB) approved all procedures, and participants signed an IRB-approved informed consent form. Participants completed a screening/training session followed by 4 randomized experimental sessions. All sessions were separated by a minimum of 48 hours and conducted at approximately the same time of day (±2 hours). All sessions began after 2 stable blood pressure readings separated by 5 minutes.
Screening and Training Session
To determine eligibility, participants completed the PAR-Q, a health history questionnaire, supplemented by clarification by interview, height and weight measurement, and a resting heart rate (HR) and blood pressure measurement. Older adults were also given a letter that had to be signed by a physician, which granted the participant medical clearance to participate in the study. The experimental sessions were not scheduled until medical clearance had been obtained. Once eligibility was determined, participants completed a training session designed to 1) teach them the continuous pain rating system and 2) determine the individualized temperatures of the thermal stimuli for the heat pain testing protocols such that participants would experience moderate pain (ie, 50/100 on a 0–100 visual analog scale). For this purpose, trains of increasing heat stimuli were applied to the forearm until participants experienced a moderate level of pain (40–60 on a 0–100 visual analog scale). In addition, the experimental pain testing procedures were conducted once during the training session to ensure familiarity with the testing protocols. During this session, participants also completed the International Physical Activity Questionnaire, which assesses the amount of time during the previous week spent on vigorous activity, moderate activity, and walking.
During the training session, maximal voluntary contraction (MVC) of handgrip muscles was also determined using a hand dynamometer (Jamar Hydraulic Hand Dynamometer; Patterson Medical, Warrenville, IL). The dynamometer handle was adjusted according to manufacturer guidelines for each participant. Participants placed their dominant arm on a table surface with the elbow at a 90° angle. Participants were asked to squeeze a hand dynamometer as hard as possible for 5 seconds. This procedure was repeated 3 times with a 1-minute rest between trials. The maximum of the 3 MVCs was used to calculate the percentage of MVC used for the isometric handgrip exercise.
Experimental Sessions
Participants completed 4 experimental sessions in randomized order consisting of 1 of the following conditions: vigorous intensity aerobic exercise, moderate intensity aerobic exercise, submaximal isometric exercise, and quiet rest. At the beginning of each session, participants were fitted with a Polar Heart Rate monitor (FT7) (Polar Electro, Lake Success, NY), which monitored and collected HR at rest (sitting) and during exercise. HR was similarly measured during all experimental sessions. During each session, 4 different pain tests were administered on each forearm followed by a 25-minute interval of aerobic exercise, quiet rest, or 22 minutes of quiet rest and a 3-minute isometric handgrip. Blood pressure was immediately taken on completion of exercise or quiet rest followed by the administration of the same 4 pain tests in the same order as the pre-exercise pain tests. The administration of the 4 pain tests before and after exercise took under 10 minutes to complete. Fig 1 shows a timeline of an experimental session.
Figure 1Timeline of procedures during the experimental sessions. The bidirectional arrows between the pressure and heat pain tests indicate that these tests were conducted in counterbalanced order. The site of pain testing alternated between left and right forearms, so that 1 arm was never tested consecutively. Participants maintained the same pain testing order for each session before and after exercise and quiet rest. The 25-minute period between the before and after pain testing included one of the following conditions: 1) quiet rest, 2) moderate intensity aerobic exercise, 3) vigorous intensity aerobic exercise, or 4) 22 minutes of quiet rest followed by a 3-minute submaximal isometric handgrip. Abbreviations: L, left forearm; R, right forearm; PPS, pressure pain suprathreshold test.
This session tested for changes in pain sensitivity and perception (as described in the section on Psychosocial Pain Testing) after 25 minutes of vigorous stationary cycling. Participants cycled the first 5 minutes at an intensity of up to 50% HR reserve (HRR) (warm-up period), followed by 20 minutes at 70% HRR. The speed and/or resistance of the cycle ergometer were adjusted to meet the prescribed intensity (target HR zone) throughout the exercise bout. The following data were recorded every 5 minutes during exercise: 1) ratings of perceived exertion (RPE) using the Borg 6–20 scale,
The Karvonen formula is related to the percent of age-predicted maximal HR but allows for differences in resting HR with the following formula: target HR = [(Age-predicted maximal HR − Resting HR) × % Intensity] + Resting HR. Age-predicted maximal HR = 220 − age.
Acute bout of moderate intensity aerobic exercise session
This session tested for changes in pain sensitivity after 25 minutes of moderate intensity stationary cycling. This session was identical to the vigorous exercise session; however, after the 5-minute warm-up period, participants cycled for 20 minutes at an intensity of 50 to 55% HRR.
Submaximal isometric exercise session (isometric)
This session tested for changes in pain sensitivity after a 3-minute trial of submaximal isometric handgrip exercise at 25% of MVC. We chose a duration of 3 minutes for the handgrip task because previous research has shown that handgrips of this duration produce the largest EIH effects (as opposed to 1- and 5-minute handgrips).
Twenty-two minutes separated the pre-exercise pain assessments and the initiation of the isometric contraction, during which participants sat quietly. The isometric handgrip exercise was performed with the dominant arm resting on the table surface with the elbow at a 90° angle. Participants were able to see the dynamometer readout and adjust their effort as necessary to maintain a level of force production at 25% of their MVC. RPE using the Borgs 6–20 RPE scale and HR were assessed every 20 seconds during the isometric exercise trial.
This session tested for changes in pain sensitivity and perception after 25 minutes of quiet rest. Participants remained in a seated position for the entire 25 minutes and were allowed to read. HR was recorded every 5 minutes.
Psychophysical Pain Testing
Participants were administered 4 different pain tests to each forearm before and after exercise or quiet rest, including 1) pressure pain thresholds (PPTs), 2) suprathreshold pressure pain trial, 3) prolonged static heat pain trial, and 4) TS of heat pain trial. The order of the pain tests is shown in Fig 1 and they were conducted as follows: 1) a pressure pain test administered to both forearms, 2) a prolonged or TS heat pain test administered to 1 forearm, 3) a prolonged or TS heat pain test administered to the other forearm, 4) a pressure pain test administered to both forearms, 5) a prolonged or TS heat pain test administered to 1 forearm, 6) a prolonged or TS heat pain test administered to the other forearm. The site of pain testing alternated between left and right arms, so that 1 arm was never tested consecutively. In addition, the order of the pressure and heat pain tests, as well as the bodily site (right vs left arm) was counterbalanced among participants. Participants maintained the same pain testing order for every session before and after exercise and quiet rest.
PPTs
PPT was assessed with a handheld algometer (Jtech, Heber City, UT) on the right and left ventral forearm, approximately 8 cm distal to the elbow. The tip of the algometer consisted of a rubber flat 1.0-cm2 probe. Pressure stimuli were delivered to the forearm at an approximate rate of .5 kg/s. Participants were instructed to respond verbally when the pressure sensation first became painful and the algometer was removed. The amount of pressure applied to the forearm did not exceed 5 kg. PPT was defined as the amount of pressure in kilograms at which the participant first reported experiencing pain.
Suprathreshold Pressure Pain Test
Ratings of suprathreshold pressure stimuli were also assessed on the right and left ventral forearm with the same handheld algometer used for PPTs. The site of the PPT and suprathreshold assessments was always separated by a minimum of 1 cm on the forearm and the same sites were used for pre- and postassessments. Pressure stimuli were delivered to the forearm at an approximate rate of .5 kg/s, until 5 kg was applied. This level of pressure was chosen to assess stimulus intensity without producing excessive discomfort. Immediately after each trial, participants rated the intensity of the stimulus on a 0 to 100 numeric rating scale (NRS), with 0 indicating “no pain” and 100 indicating “intolerable pain.”
Prolonged Static Heat Pain Test
Contact heat stimuli were delivered by a computer-controlled Peltier-based thermode (32 mm × 32 mm; TSA-2001, Ramat Yishai, Israel) to the right and left ventral forearms. For each 30-second continuous heat pain trial, the thermode was first brought to a neutral temperature (32°C) and then ramped (2.0°C/s) to the individualized temperature (44–49°C) determined during the training session and maintained at that temperature for 30 seconds. The thermode position was altered slightly between each heat trial (ie, continuous and TS heat pain trials). The intensity of the pain produced by the contact thermode was rated every 5 seconds on a 0 to 100 NRS. As performed in previous studies using prolonged static heat tests, the area under the curve (AUC) was calculated for each trial by summing the recorded pain ratings.
AUC was chosen as the primary dependent variable for this test because it has been used in past research as an outcome variable for prolonged heat pain ratings and is an effective way to simply quantify the amount of pain experienced over time.
Brief repetitive suprathreshold heat pulses were delivered to the right and left ventral forearms. Each trial consisted of a series of 10 heat pulses, with each pulse delivered at a rate of 10°C/s. The peak-to-peak interpulse interval was approximately 2.5 seconds. The baseline temperature was 38°C and the target temperature was the individualized temperature determined during the training session (45–51.5°C). Participants were instructed to rate the intensity of the late pain sensations experienced after each pulse (ie, pain felt between the pulses not during each pulse, termed second pain) with a 0 to 100 scale. This pain test permitted the assessment of the effect of exercise on the TS of C-fiber-mediated heat pain (ie, late heat pain sensations, often termed second pain).
A TS score was calculated by subtracting the pain rating after the first pulse from the highest interpulse pain rating. This score captures the maximum amount of TS across the 10 pulses.
Reliability of Pain Measures
Because we administered only 1 trial at each body site for each pain test at pretest and posttest, we conducted interclass correlations coefficients (ICCs) on the pretests for each pain measure to determine the reliability of these measures. The ICCs were conducted separately for older and younger adults. These analyses indicated excellent reliability for all pain measures for younger adults (ICCs ranged from .80 to .93) and older adults (ICCs ranged from .82 to .92).
Data Analysis
Descriptive statistics were calculated for average percentage of HRR, and RPE for each exercise session. One younger participant's average HRR% for the vigorous aerobic exercise was only 55%; therefore, this participant's data were not included in the statistical analyses. During the suprathreshold pressure pain test, 1 younger participant did not report experiencing any pressure pain after the target pressure of 5 kg was reached; thus, this participant's data were removed from the suprathreshold pressure pain analyses. During the PPT test, 2 younger participants did not report experiencing pain before the upper limit of pressure for the test was reached; therefore, their data were not included in the PPT analyses.
The primary purpose of this study was to determine whether EIH differed by age. Thus, we created an index of EIH for each pain test, similar to what has been used in other pain inhibitory tests such as CPM.
First, change scores for each dependent variable were calculated for each session by subtracting the pretest value from the posttest value. Then, the control change score was subtracted from the exercise session (isometric, moderate aerobic exercise, or vigorous aerobic exercise) change scores (EIH index = [Posttest score exercise − Pretest scoreexercise] − [Posttest scorecontrol − Pretest scorecontrol]). The adjusted change score (EIH index) provides a controlled measure of the degree to which pain perception changed as a function of the exercise. A positive number for the PPTs indicates that pain sensitivity was reduced after exercise compared with the control condition. For the other 3 measures, a negative value indicates that pain was reduced after exercise compared with the control condition. A Shapiro-Wilk test of normality was used to test for normal distribution of each measure in each age group.
To determine age differences in EIH during the isometric exercise session, the EIH index for each pain test was analyzed with an Age group × Sex × Forearm (active vs inactive) mixed model analysis of covariance (ANCOVA) with target force level added as a covariate. Thermode temperature was added as a covariate for the heat pain tests. In addition, preliminary analyses showed that PPTs differed between age groups at baseline; therefore, the average pretest PPT score was also added as a covariate for the PPT analyses. Sex was included as a factor because previous research has shown sex differences in EIH.
Forearm was included as a variable to determine whether EIH differed between the exercised and nonexercised forearm.
For the aerobic exercise sessions, data for each pain test were averaged between the 2 forearms. To determine age differences in EIH during aerobic exercise, the EIH index for each pain test was analyzed with an Age group × Sex × Session (moderate vs vigorous) mixed model analysis of variance (ANOVA). Thermode temperature was added as a covariate for the heat pain tests. Preliminary analyses showed that PPTs differed between age groups at baseline; therefore, the average pretest PPT score was also added as a covariate for the PPT analyses. If the sphericity assumption was violated, then Greenhouse-Geisser degrees of freedom corrections were applied to obtain the critical P value. Post hoc comparisons were made with the Tukey honest significant difference procedure. A level of P ≤ .05 was used for all statistical analyses.
To determine the magnitude of age differences in EIH, ESs were calculated using Cohen's d. Cohen's d was defined as the mean for the young adults minus the mean for the older adults, divided by the pooled within-group standard deviation (d = [Xyoung − Xold]/Pooled standard deviation). ESs were calculated for men and women separately. A positive ES reflects greater EIH for the younger adults. These ESs are presented in Table 1.
Table 1EIH Index Means and SE and ESs for Each Pain Test and Exercise Session
NOTE. A positive number for the PPTs indicates that pain sensitivity was reduced after exercise compared with the control condition. For the other 3 measures, a negative value indicates that pain was reduced after exercise compared with the control condition. The ESs represent the magnitude of difference between older and younger adults. A positive ES indicates that EIH was greater for younger adults compared with older adults.
ESs were also calculated to determine the magnitude of pain reduction after exercise. Because we did not find differences between forearms, data for each pain test were averaged between the 2 forearms. Cohen's d was defined as the mean for trial 1 minus the mean for trial 2, divided by the pooled within-group standard deviation (d = [Xtrial1 − Xtrial2]/Pooled standard deviation). Because of the within-subjects design, the ESs were adjusted as recommended by Portney and Watkins.
AUC values by sex for isometric condition: young male pre, 83.10 ± 22.7, post, 183.12 ± 25.4; young female pre, 205.16 ± 24.3, post, 165.87 ± 23.8; older male pre, 148.86 ± 31.6, post, 136.93 ± 31.0; older female pre, 258.69 ± 37.9, post, 195.19 ± 37.8.
NOTE. ESs represent the magnitude of change from pretest to posttest. A positive ES indicates that pain was reduced after exercise or quiet rest.
∗ AUC values by sex for isometric condition: young male pre, 83.10 ± 22.7, post, 183.12 ± 25.4; young female pre, 205.16 ± 24.3, post, 165.87 ± 23.8; older male pre, 148.86 ± 31.6, post, 136.93 ± 31.0; older female pre, 258.69 ± 37.9, post, 195.19 ± 37.8.
Two-way ANOVAs determined whether differences existed between age groups and sex on thermode test temperatures and target force production. The Shapiro-Wilk test of normality indicated that the International Physical Activity Questionnaire data were not normally distributed; thus Mann-Whitney U tests were conducted to determine if the IPAQ scores differed by age. Average HRR% during the aerobic exercise sessions was analyzed with a 3-way ANOVA with sex and age group as between-subjects factors and session as the within-subject factor. Average RPE during all exercise sessions was also analyzed with an Age group × Sex × Session ANOVA. All data presented in the text are presented as means ± standard error (SE).
Results
The EIH index means and SEs for each pain test and exercise session by sex and age group are presented in Table 1. In addition, the means and SEs for the pretest and posttest values for each pain test and condition by age group are presented in Table 2. See Table 3 for a summary of significant age and sex differences in EIH.
Table 3Summary of Significant Age and Sex Differences for Each Exercise Condition and Pain Test
Pain Test
Isometric
Moderate AE
Vigorous AE
PPT
Age
Age
Age
PPS
None
Age
None
TS maximum
Age
None
None
AUC heat pain
Sex
Age
Age
Abbreviations: AE, aerobic exercise; Age, significant age differences; None, no significant differences between groups; PPS, pressure pain suprathreshold test (significance P < .05); Sex, significant sex differences.
The 3-way ANCOVA conducted on the EIH index for PPTs revealed a main effect of age group (P = .030). Younger adults experienced greater EIH compared with the older adults (younger adults, .24 ± .09; older adults, −.12 ± .12). The ESs indicated a small age group difference for females and a large difference for males. No other main effects or interactions were significant (P > .05). The ESs presented in Table 2 show that the magnitude of pain reduction was moderate to small for younger adults and very small for older adults.
The ANCOVA conducted on the suprathreshold pressure pain EIH index showed no significant results (P > .05). ESs indicated that the magnitude of pain reduction after isometric exercise was small for younger adults and virtually nonexistent for older adults.
Heat Pain Tests Results
The analysis on the EIH index for TS of pain revealed significant age differences (P = .01), with younger adults exhibiting greater EIH compared with older adults (younger adults, −4.99 ± 1.80; older adults, 2.78 ± 2.06). No other main effects or interactions were significant (P > .05). The ESs indicated a moderate age group difference for males and a large difference for females. The magnitude of pain reduction for younger adults was moderate, whereas the ES for older adults revealed a small increase in TS of pain after exercise.
The ANCOVA conducted on the EIH index for AUC on the continuous heat pain test revealed a significant main effect of sex (P = .043). Females showed greater EIH compared with males (females, −28.43 ± 18.20; males, 40.49 ± 20.00), regardless of age. In regards to magnitude of pain reduction after isometric exercise, the ESs were as follows: younger males, ES = .0004; older males, ES = .21; younger females, ES = .63; older females, ES = .84. No other main effects or interactions were significant (P > .05).
Aerobic Exercise
Pressure Pain Test Results
Similar to the isometric exercise results, the 3-way ANOVA conducted on the EIH index for PPT showed a significant effect of age group (P = .046). Younger adults exhibited greater EIH after aerobic exercise compared with older adults (younger adults, .29 ± .09; older adults, −.06 ± .11). No significant differences were found as a function of session (moderate vs vigorous exercise) or sex. The magnitude of age differences (Table 1) was moderate for the moderate intensity aerobic exercise and large for vigorous aerobic exercise. The magnitude of pain reduction after moderate aerobic exercise (Table 2) was small for younger and older adults. For vigorous aerobic exercise, the magnitude of pain reduction was moderate for young adults and small for older adults. Importantly, ESs showed that the magnitude of pain reduction for older adults was greater during the control condition compared with the exercise sessions.
A significant Age group × Session interaction (P = .028) was found for the EIH index for suprathreshold pressure pain. Older adults exhibited reduced EIH after moderate aerobic exercise compared with vigorous aerobic exercise and compared with younger adults after moderate aerobic exercise (moderate exercise, younger adults, −6.10 ± 4.03; vigorous exercise, younger adults, −4.09 ± 3.70; moderate exercise, older adults, .97 ± 5.06; vigorous exercise, older adults, −6.23 ± 4.66). Accordingly, the ESs showed that the magnitude of age differences was moderate to large for the moderate aerobic exercise and small to nonexistent for the vigorous aerobic exercise. However, the magnitude of pain reduction on the suprathreshold pain test after aerobic exercise was small for both age groups, ranging from −.28 to .16 (Table 2). No other main effects or interactions were significant (P > .05).
Heat Pain Tests Results
The analysis on the EIH index for TS of pain revealed no significant results (P > .05). The EIH index for younger and older adults by session was as follows: moderate exercise, younger adults, −3.05 ± 1.50; vigorous exercise, younger adults, .25 ± 1.60; moderate exercise, older adults, .03 ± 2.03; vigorous exercise, older adults, −1.4 ± 2.18. Generally, the ESs in Table 2 indicate that the magnitude of reduction in TS of pain after aerobic exercise was small for both groups, ranging from .37 to −.11.
The 3-way ANOVA conducted on the EIH index for AUC for the continuous heat pain test showed a significant effect of age group (P = .001), with younger adults exhibiting greater EIH compared with older adults, regardless of the session (younger adults, −19.90 ± 11.0; older adults, 41.67 ± 13.83). The magnitude of the age differences was large for males and females after vigorous exercise and for females after moderate exercise, but small for males after moderate aerobic exercise. Although the older adults revealed a moderate level of pain reduction after aerobic exercise (Table 2), the magnitude of pain reduction during the control session was greater.
Characteristics of Exercise and Pain Tests
Table 4 presents the means and SE for each age group by sex for target force during the isometric handgrip, average HRR% during the aerobic exercise sessions, average RPE during all exercise sessions, and average thermode temperature for the heat pain tests. The analysis conducted on target force during the isometric handgrip showed a main effect of age group (P = .002) and sex (P < .001). Males had greater force production than females during the handgrip and younger adults produced greater force than older adults. The analyses on HRR% confirmed that participants exercised at a greater HRR% during the vigorous aerobic exercise (M = 70.84 ± 1.28) compared with the moderate aerobic exercise (M = 53.35 ± .71). HRR% did not differ as a function of sex or age group (P > .05). The ANOVA conducted on RPE revealed a main effect of exercise session (P < .001). For all participants, RPE was greater during the vigorous aerobic exercise compared with moderate aerobic exercise and the isometric exercise. The 2-way ANOVAs conducted on thermode temperature for the heat pain tests revealed no significant differences between age groups or sex (P > .05). In addition, results of the Mann-Whitney U test indicated no differences between age groups in total physical activity (young, 3428 ± 494 METS-min/wk; older, 5368 ± 1330 METS-min/wk), vigorous physical activity (young, 1393 ± 248 METS-min/wk; older, 1910 ± 555 METS-min/wk), moderate physical activity (young, 852 ± 1030 METS-min/wk; older, 1927 ± 654 METS-min/wk) and walking (young, 1182 ± 279 METS-min/wk; older, 1430 ± 512 METS-min/wk) on the IPAQ.
Table 4Descriptors of Exercise and Thermode Test Temperature (Means ± SE)
The current study investigated age differences in EIH after isometric and aerobic exercise using heat and pressure psychophysical testing. Three key findings emerged from this study: 1) age differences in EIH emerged after isometric and aerobic exercise, with younger adults experiencing greater EIH compared with older adults, 2) the age differences in EIH varied across pain induction methods, and 3) despite the observed reduction in EIH for older adults, these participants generally did not show increased pain perception or sensitivity after acute exercise.
Age Differences in EIH After Isometric Exercise
Supporting our hypothesis, we found age differences in EIH during the submaximal isometric handgrip condition with the PPT test. PPTs signify the lowest boundary of painful sensations in musculoskeletal structures.
The degree to which isometric exercise increased the pain threshold boundary was greater for younger compared with older adults. This finding is in partial support of Lemley and colleagues,
who found increased PPTs after isometric exercise for older and younger adults; however, the magnitude of EIH was greater in younger adults. In contrast to the PPT data, our results did not show age differences in EIH on the suprathreshold pressure pain test. The lack of age differences was likely caused by the negligible effect of the isometric handgrip on pain ratings during the pressure suprathreshold test in all participants. The isometric exercise did not cause a hyperalgesic response in older adults on either pressure pain test.
The current study was the first to examine EIH in older adults using TS of pain and prolonged heat pain. TS of pain is reduced in younger adults after isometric exercise
Our findings revealed that an isometric handgrip induces greater EIH on the TS test in younger compared with older adults. ESs revealed a moderate reduction in TS in younger adults and a minimal to small increase in older adults.
Using the prolonged heat pain test, we discovered sex rather than age differences in EIH, with females experiencing greater EIH compared with males. Excluding the difference in modality (pressure vs heat), the prolonged suprathreshold heat test used in the current study was similar to the prolonged suprathrehold pressure pain test used in the study by Lemley et al, which also found sex rather than age differences in EIH.
The reason for the sex differences in EIH during prolonged static pain tests remains unclear. Hashmi and Davis suggested that effective central inhibitory mechanisms to attenuate sustained pain would be more biologically advantageous for women to cope with natural pain, including childbirth pain.
Nonetheless, the mechanism enabling women effective isometric EIH for prolonged suprathreshold pain does not appear to deteriorate with age.
Age Differences in EIH After Aerobic Exercise
To the best of our knowledge, this is the first study to investigate age differences in EIH after aerobic exercise. Previous research has shown that aerobic exercise reduces pain perception in healthy adults but can have no effect or even a hyperalgesic effect on experimentally induced pain in patients with chronic pain.
In the current study, moderate and vigorous aerobic exercise elicited greater EIH for the PPT test in younger compared with older adults. The data also showed age differences in EIH for the suprathreshold pressure pain test. Older adults exhibited less EIH compared with younger adults only during the moderate aerobic exercise condition. The magnitude of change in pain ratings on the suprathreshold pressure pain test was minimal to small for all exercise conditions and groups (ie, ESs range from .16 to −.28). The small effects of exercise on the suprathreshold pressure pain test may have been caused by the large between-subject variability in pre-exercise pain ratings during this test (ie, ranging from 5 to 99), increasing the likelihood of ceiling and floor effects.
We also found age differences in aerobic EIH with the prolonged heat pain test. Although older men and women showed a reduction in pain ratings after aerobic exercise on this test, the reduction in pain ratings from pre- to posttest was greater during the control session. Thus, although aerobic exercise did not induce a hypoalgesic response compared with quiet rest, heat pain perception was also not enhanced by exercise as found in some patients with chronic pain.
In contrast to our hypothesis, we observed no age differences in aerobic EIH using the TS test. The small magnitude of change in TS of pain after aerobic exercise for both age groups may have contributed to the lack of age differences using this measure. In a previous study using the same repetitive pulse heat pain test as the current study, Naugle et al
found that aerobic exercise decreased pain ratings on pulses in the latter end of the TS trial (ie, pulses 6–10) in younger adults. However, the study by Naugle et al did not look at the effect of aerobic exercise on the magnitude of summation (increase in pain ratings from first pulse to maximum pain rating) during the TS trial. Perhaps, aerobic exercise temporarily reduces heat pain sensitivity in healthy younger adults but does not reduce the hyperexcitability of the central nervous system.
Several different mechanisms have been proposed to underlie EIH and could underlie the deterioration of this phenomenon with age. The most widely ascribed mechanism for EIH involves the activation of the endogenous opioid system during exercise. Animal studies indicate that exercise of sufficient intensity and duration causes the release of central and peripheral β-endorphins, which have been linked with decreased pain sensitivity.
suggested the involvement of a nonopioid versus opioid mechanism in isometric EIH using TS and PPTs as the experimental pain tests. Greater EIH was associated with increased circulating levels of the endocannabinoid docosahexaenoylethanolamine (DHA) after isometric exercise. DHA deficiency is associated with aging
Doxosahexaenoic acid signalolipidomics in nutrition: significance in aging, neuroinflammation, macular degeneration, Alzheimer's, and other neurodegenerative diseases.
; however, age-related differences in the DHA response to exercise is not known. Another potential mechanism involves the activation of endogenous pain inhibitory mechanisms, such as CPM. CPM refers to the phenomenon whereby a noxious stimulus at one body part results in reduced pain perception to another noxious stimulus at a distant body part.
recently tested this hypothesis by examining EIH via painful exercise, nonpainful exercise, and quiet rest. The results suggested that although exercise-induced muscle pain may contribute to the magnitude of pain reduction after acute exercise, CPM is likely not the primary mechanism of EIH. Lemely et al
tested whether CPM predicts isometric EIH in healthy older and younger adults. Findings revealed that individuals exhibiting a greater ability to activate descending inhibitory pathways in the CPM paradigm also showed greater EIH. Thus, although multiple factors likely contribute, abnormal descending pain inhibition may play a role in the diminished hypoalgesic effect of acute exercise in older adults. Although the mechanisms underlying age differences in EIH were not tested in this study, our results suggest that age-related reductions in EIH after isometric and aerobic exercise are likely caused by unique and shared mechanisms. For example, with the prolonged heat test used as the test stimulus, we observed age differences in EIH during the aerobic conditions but sex differences in EIH during the isometric condition. These findings illustrate the complexity of the EIH phenomenon, which is likely produced by multiple analgesic systems, each of which may preferentially alter different types of nociceptive input.
A few limitations of this study should be noted. First, the older adult group in this study was healthy and active. Thus, these results may not generalize to older adults who are less active or present with more health conditions. Second, we did not assess for potential postexercise pain. Participants who completed sessions separated by only 48-hours may have experienced delayed onset-muscle soreness (DOMS) in the latter session. However, given the activity level of participants, the risk for DOMS was likely low. Based on our power analysis, this study was not powered to detect small effects.
The present data suggest diminished EIH in older adults compared with younger adults after isometric and aerobic exercise. However, our data in combination with Lemley et al, also suggest that acute exercise generally does not cause a hyperalgesic response in healthy older adults. Our results also showed that EIH varies by age based on the pain test stimulus and type of exercise. Little is known regarding the clinical implications of experiencing diminished EIH and which pain test/modality in testing EIH has the most clinical relevance. Future studies need to investigate the impact of individual differences in EIH on physical activity behavior and clinical pain experiences specifically related to physical activity in older adults. This knowledge could have important implications regarding the identification of high-risk individuals for persistent pain, declining physical activity levels, and functional disability.
References
Bazan N.G.
Molina M.F.
Gordon W.C.
Doxosahexaenoic acid signalolipidomics in nutrition: significance in aging, neuroinflammation, macular degeneration, Alzheimer's, and other neurodegenerative diseases.
This research was supported by NIH-NIA Grant R01AG039659 (J.L.R.) and NIH Grant T32 T32NS045551-06 (K.M.N.). There are no other conflicts of interest to report with regard to this work for any of the authors.
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