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An Improved Rodent Model of Trigeminal Neuropathic Pain by Unilateral Chronic Constriction Injury of Distal Infraorbital Nerve

  • Weihua Ding
    Affiliations
    Department of Anesthesia, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China

    Hangzhou First People's Hospital, Hangzhou, China
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  • Zerong You
    Affiliations
    MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Shiqian Shen
    Affiliations
    MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Jinsheng Yang
    Affiliations
    MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Grewo Lim
    Affiliations
    MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Jason T. Doheny
    Affiliations
    MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Lucy Chen
    Affiliations
    MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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  • Shengmei Zhu
    Correspondence
    Dr. Shengmei Zhu, MD, Department of Anesthesia, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou 310003, China.
    Affiliations
    Department of Anesthesia, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
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  • Jianren Mao
    Correspondence
    Address reprint requests to Dr. Jianren Mao, MD, PhD,MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114.
    Affiliations
    MGH Center for Translational Pain Research, Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts
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Published:February 23, 2017DOI:https://doi.org/10.1016/j.jpain.2017.02.427

      Highlights

      • We improved surgical procedure to induce trigeminal neuropathic pain in rats.
      • We performed chronic constriction injury on distal infraorbital nerve (dIoN-CCI).
      • The dIoN-CCI model was easy to perform, minimally invasive, and time saving
      • The dIoN-CCI rats exhibited pain behaviors over a prolonged period.
      • This is a more clinically relevant rodent model for trigeminal neuropathic pain.

      Abstract

      The number of studies on trigeminal nerve injury using animal models remains limited. A rodent model of trigeminal neuropathic pain was first developed in 1994, in which chronic constriction injury (CCI) is induced by ligation of the infraorbital nerve (IoN). This animal model has served as a major tool to study trigeminal neuropathic pain. Unfortunately, the surgical procedure in this model is complicated and far more difficult than ligation of peripheral nerves (eg, sciatic nerve). The aim of this study was to improve on the current surgical procedure of IoN ligation to induce trigeminal neuropathic pain in rats. We show that the IoN can be readily accessed through a small facial incision. CCI can be induced by ligation of a segment at the distal IoN (dIoN). This dIoN-CCI procedure is simple, minimally invasive, and time-saving. Our data show that the dIoN-CCI procedure consistently induced acute as well as chronic nociceptive behaviors in rats. Daily gabapentin treatment attenuated mechanical allodynia and reduced face-grooming episodes in dIoN-CCI rats.

      Perspective

      The orofacial pain caused by trigeminal nerve damage is severe and perhaps more debilitating than other types of neuropathic pain. However, studies on trigeminal neuropathic pain remain limited. This is largely because of the lack of proper animal models because of the complexity of the existing surgical procedures required to induce trigeminal nerve injury. Our improved dIoN-CCI model is likely to make it more accessible to study the cellular and molecular mechanisms of neuropathic pain caused by trigeminal nerve damage.

      Key words

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