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The spinal cord dorsal horn is a key area for the processing of sensory information
such as pain, heat, cold, touch and itch. Our understanding of spinal cord microcircuitry
has proved difficult, in part, due to a lack of effective tools to allow identification
of dorsal horn neuron populations and how they are connected. A recent technique developed
in our lab using pharmacology-based profiling (termed CICADA) has allowed us to visualize
eight populations of excitatory neurons in the superficial dorsal horn. Here, we use
two-photon Ca2+ imaging and CICADA to tease apart connectivity across these CICADA-defined
populations. Through back labeling spinoparabrachial projection neurons, we also identify
spinal output neurons, and the spinal networks that provide input onto these cells.
These experiments reveal the network connectivity, including the spinal output neurons,
that are downstream of each CICADA-defined population. Together, these findings provide
new insight into the connectivity in the superficial dorsal horn. Grant support from
2R01 NS096705-06.
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© 2022 Published by Elsevier Inc.
